Symbol N
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A useful way to see the tradeoff quantitatively is coverage. If a screen library contains g guides and the experimenter wants, on average, c cells carrying each guide to survive Poisson dropout during selection, the number of cells that must be carried through the pipeline scales roughly as . where is the expected number of guide integrations per cell under the multiplicity of infection chosen for the transduction (kept low, near 0.3, specifically so that most transduced cells receive at most one guide). Doubling library size to cover more genes, or increasing per-guide coverage to detect weaker effects, both push cell numbers up directly — which is exactly why screen…
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Symbol g
g is a part of this expression. Its role is fixed by the surrounding article and by the operations shown in the formula.
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c is a part of this expression. Its role is fixed by the surrounding article and by the operations shown in the formula.
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u is a part of this expression. Its role is fixed by the surrounding article and by the operations shown in the formula.
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Equation 3 · Biology
Comparing the Main Approaches to Molecular Biology and Genomics
This mathematical expression combines the displayed quantities; its precise role follows from the surrounding article text.
A useful way to see the tradeoff quantitatively is coverage. If a screen library contains g guides and the experimenter wants, on average, c cells carrying each guide to survive Poisson dropout during selection, the number of cells that must be carried through the pipeline scales roughly as . where is the expected number of guide integrations per cell under the multiplicity of infection chosen for the transduction (kept low, near 0.3, specifically so that most transduced cells receive at most one guide). Doubling library size to cover more genes, or increasing per-guide coverage to detect weaker effects, both push cell numbers up directly — which is exactly why screen…
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